RealHelix™ Premier qPCR Kit [Green] 은 특이도(specificity)와 민감도(sensitivity)가 높고 target DNA를 빠르게 정량 분석할 수 있는 real-time PCR 제품입니다. 본 제품에 적용된 나노헬릭스만의 독특한 buffer system과 antibody-mediated hot start enzyme은 primer dimer 생성을 최소화하고, 결과의 신뢰도를 높여줍니다. 제공되는 2x 농도의 premix에는 Ab+Taq polymerase, dNTPs, buffer, Mg²⁺, green fluorescent dye 및 안정제(stabilizers)가 혼합되어 있습니다.

Figure 1. Sensitive detection using RealHelix™ Premier qPCR Kit [Green, Low ROX].

A standard sample of 10-fold serial-diluted genomic DNA was analyzed using the RealHelix™ Premier qPCR Kit for the human glucose-6-phosphate dehydrogenase gene-specific primer set. The accuracy of real-time PCR using the RealHelix™ Premier qPCR Kit is demonstrated by R² value=0.99887. NTC: Negative control.

Figure 2. Amplification of target DNA with various G+C contents using RealHelix™ Premier qPCR Kit [Green, Low ROX].

It was evaluated by real-time PCR using 10-fold serial diluted human genomic DNA (red: 50 ng/rxn, green: 5 ng/rxn, and blue: 0.5 ng/rxn) with each instrument. NTC: Negative control.

Figure 3. Comparison of real-time PCR using RealHelix™ Premier qPCR Kit [Green, Low ROX] with other company's related products.

10-fold serial dilutions of the human genomic DNA (red: 50 ng/rxn, green: 5 ng/rxn, and blue: 0.5 ng/rxn) from whole blood were analyzed using the human HPRT-1 gene-specific primer set. Reactions were run on a real-time PCR instrument, CFX-96 (Bio-Rad, USA). NTC: Negative control.

Storage

• Store below -20 ℃
• Should be stored in a dark place.

Shelf life

• 12 months